Determination of Maillard Reaction markers in doce de leite by HPLC-PDA

Caroline Barroso dos Anjos Pinto¹, Carolina Neves Cunha², Natalia Casas Mesa¹, Maria Esther Leal da Silva Sad², Rodrigo Stephani¹, Ítalo Tuler Perrone², and Juliana de Carvalho da Costa²

¹Departamento de Química, Universidade Federal de Juiz de Fora, Juiz de Fora – MG, Brasil

²Departamento de Ciências Farmacêuticas, Universidade Federal de Juiz de Fora, Juiz de Fora – MG, Brasil

Abstract:

This study was carried out as a preliminary mapping of Maillard reaction (MR) compounds in doce de leite (DL), as possibleindicators for the intermediate stage and chemical pathways in industrial processing. For that, the objective of the manuscript was to developed and validated an analytical method to simultaneously analyze four furfural compounds in DL: 5-hydroxymethylfurfural, 2-furaldehyde (F), 2-furyl-methyl ketone (FMC) and 5-methyl-2-furaldehyde (MF) by high performance liquid chromatography-photodiode-array detector (HPLC-PDA). The method fulfilled the acceptance criteria: selectivity, linearity (0.16‑5 μg mL-1 for HMF, and 0.06-2 μg mL-1 for F, FMC and MF, R2 > 0.9974), precision (CV = 1.33 to 2.12% for HMF; 1.57 to 4.34% for F; 0.84 to 1.40% for FMC and 1.40 to 4.18% for MF), accuracy (94.40 to 102.25% for HMF; 92.91 to 108.15% for F; 90.13 to 108.48% for FMC and 93.31 to 107.70% for MF), limit of detection of 0.041 μg mL 1 for HMF; 0.030 μg mL-1 for F; 0.042 μg mL-1 for FMC and MF, and limit of quantification of 0.125 μg mL-1 for HMF and F, 0.066 μg mL-1 for FMC and 0.1280 μg mL-1 for MF. Thus, it was concluded that an analytical method was developed and validated for quantifying HMF, F, FMC and MF in DL.

Keywords: validation; sugars; methodology; chromatography; furfurals.

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